![]() ![]() Static Light Scattering involves measuring the scattering intensity of a number of concentrations of the sample from which the molecular weight of proteins and polymers and the second virial coefficient can be determined which provides a measure of protein solubility.ĪTA Scientific offers a range of particle sizing instruments that employ all three types of light scattering technologies – dynamic, static and electrophoretic light scattering. An electric field is applied to a solution of molecules or a dispersion, which causes the particles to move at a certain speed from which mobility and zeta potential is calculated. Micro-electrophoresis combined with Phase Analysis Light Scattering (M3-PALS) is used to measure both the zeta potential of dispersions and surface zeta potential of a particle to determine formulation stability. Then a second mathematical analysis known as a ‘polydispersity analysis’ is performed on the correlation function to determine the size of particles and the relative numbers of each size. Fluctuations in the intensity of scattered light are measured for a period of time and then the data is processed into a ‘correlation function’ (a mathematical function that identifies the patterns). DLS monitors Brownian Motion with light scattering. The bigger the particle, the slower the Brownian motion will be. Dynamic light scattering (DLS) is a technique for determining particle size by measuring the Brownian motion (the random movement) of particles in a suspension or solution. ![]()
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